Journal of Ecophysiology and Occupational Health

H. K. Singh ¹

Affiliations
1 Department of Zoology, T. D. College, Jaunpur (UP), IN

Abstract

The aim of this investigation was to know daily changes (circadian) in the blood plasma free (unconjugated) testosterone (TF) and estradiol-17β(E2F) and its conjugated testosterone glucuronide (TG) and testosterone sulfate (TS) as well as estradiol-17β glucuronide (E2G) and estradiol-17β sulfate (E2S) sex steroids in a mature female fish Cirrhinus mrigala (Ham.) during spawning phase using enzyme-linked immunosorbent assay (ELISA) method. The two sex steroids testosterone and estradiol-17β exhibited identical circadian rhythm: a major peak 29.02±3.58 ng/ml occurred at the onset of the dark phase and a minor peak 12.79±1.89 ng/ml was generally observed 4 hour after the onset of light phase . The TG peak (9.23±1.04 ng/ml was in the dark phase whereas TS during 12.00 PM of dark phase. The E2G 7.19±.76 ng/ml peak was also indicated high during 04.00 am of the dark phase. Result indicated that during the dark phase elevation of free and conjugated sex steroids have important role for spawning, and maintaining the equilibrium of free and conjugated steroids.

Keywords

Circadian, Sex Steroid, Glucuronide, Sulfate, Hormones, Fish, Cirrhinus mrigala.

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P. B. Singh ¹, S. K. Nigam ¹, Rajveer Singh ¹, H. K. Singh ¹

Affiliations
1 Ganpat Sahai PG College, Sultanpur-228001, IN

Abstract

The objectives of the present investigation was to monitor the HCHs (isomers of hexachlorocyclohexane), DDTs (metabolites of dichlorodiphenyltrichloroethane), chlorpyrifos in tissue bioaccumulation and plasma levels of cortisol between the sampling sites of the unpolluted ponds of Gujartal, Jaunpur (reference site) and the polluted rivers the Gomti (Jaunpur) and the Ganga (Varanasi) as stress hormone of some edible catfishes during non-breeding season owing to industrialization. The insecticides were measured by the gas liquid chromatography (GLC) and plasma cortisol by Enzyme-Linked Immunosorbents Assay (ELISA). The results indicated that the presence of HCHs, DDTs and chlorpyrifos was much higher in the catfishes captured from the river Gomti than those of the river Ganga when compared to reference site. The plasma level of cortisol was low in the fishes captured from both the rivers. In conclusion, the fishes from polluted rivers showed high degree of contamination which caused stress and ultimately decreased the growth of edible fishes of riverine sources.

Keywords

Insecticides, Bioaccumulation, Wild Catfishes, Cortisol, Stress Hormone, River Pollution.

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